Dual-label STED nanoscopy of living cells using photochromism.
نویسندگان
چکیده
We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.
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عنوان ژورنال:
- Nano letters
دوره 11 9 شماره
صفحات -
تاریخ انتشار 2011